IMMUNOLOGY R&D SERVICES
No vaccine candidate can move ahead without a comprehensive analysis of its immunology, and sterility. At TM3, we offer a complete range of services to further your program. Our comprehensive immunology services include:
Immune Response Measurements
HUMORAL IMMUNE RESPONSES
• Antibody titers
• ELISA
• Neutralization assays
• ADCC activity
• Western blots
CELL MEDIATED IMMUNE RESPONSE ASSAYS
• ELISPOT for single cell activities
• Intracellular Cytokine Staining and ELISA measuring cytokines
• CD4 antigen specific proliferation assays
• Mixed lymphocyte reaction assays
• CD8 cytotoxic activity
CELLULAR FUNCTION ASSAYS
• Chemokine analysis by chemotaxis or calcium flux assays
• Innate Immune responses specifically gamma delta T-cells and NK cells s
• Toll-like receptor activation assays
• Antigen uptake, processing and presentation
Services supporting Host pathogen R&D
• Large-scale human lymphocyte activation
• Viral and microbial assays for BSL-2 pathogens
• Real-time PCR for a quantization of a range of pathogens
• Generation of antigen-specific T-cell clones by long term culture protocols
• Monocyte preparation from whole blood cells and maturation of monocytes into macrophages
and dendritic cells
• T-cell preparation from whole blood cells, and cord blood cells using magnetic beads or FACS
• B-cell preparation from whole blood cells, and cord blood cells using magnetic beads or FACS
• Stem cells from cord blood
FLOW CYTOMETRY SERVICES
• Detection of various fluorescent protein
• Surface and intracellular cytokine staining
• Measurement of apoptosis
• Cellular phenotypic analysis
• Measurement of activation status
VACCINE EFFICACY PARAMETERS
• CD4+ and CD8+ T-cell-mediated responses
• Proliferation
• Antibody response
• Qualitative or quantitive ELISA
• Chemifluorescent immunoblotting
• Potency assay
STERILITY ASSAYS
• USDA and FDA Standards
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