TM3 THERAPEUTICS ADDRESS: 3521 INVESTMENT BLVD SUITE I HAYWARD, CA 94545
PHONE: 510-363-3748 FAX:510-266-0087
Copyright © 2010 TM3 Therapeutics EMAIL:info@tm3therapeutics.com
           

IMMUNOLOGY R&D SERVICES

No vaccine candidate can move ahead without a comprehensive analysis of its immunology, and sterility. At TM3, we offer a complete range of services to further your program. Our comprehensive immunology services include:

                                          Immune Response Measurements

HUMORAL IMMUNE RESPONSES

   • Antibody titers
   • ELISA
   • Neutralization assays
   • ADCC activity
   • Western blots
 

CELL MEDIATED IMMUNE RESPONSE ASSAYS

   • ELISPOT for single cell activities
   • Intracellular Cytokine Staining and ELISA measuring cytokines
   • CD4 antigen specific proliferation assays
   • Mixed lymphocyte reaction assays
   • CD8 cytotoxic activity
 

CELLULAR FUNCTION ASSAYS

   • Chemokine analysis by chemotaxis or calcium flux assays
   • Innate Immune responses specifically gamma delta T-cells and NK cells s
   • Toll-like receptor activation assays
   • Antigen uptake, processing and presentation

                                       Services supporting Host pathogen R&D

   • Large-scale human lymphocyte activation
   • Viral and microbial assays for BSL-2 pathogens
   • Real-time PCR for a quantization of a range of pathogens
   • Generation of antigen-specific T-cell clones by long term culture protocols
   • Monocyte preparation from whole blood cells and maturation of monocytes into macrophages   
     and  dendritic cells
   • T-cell preparation from whole blood cells, and cord blood cells using magnetic beads or FACS
   • B-cell preparation from whole blood cells, and cord blood cells using magnetic beads or FACS
   • Stem cells from cord blood

FLOW CYTOMETRY SERVICES

   • Detection of various fluorescent protein
   • Surface and intracellular cytokine staining
   • Measurement of apoptosis
   • Cellular phenotypic analysis
   • Measurement of activation status

VACCINE EFFICACY PARAMETERS
   • CD4+ and CD8+ T-cell-mediated responses
   • Proliferation
   • Antibody response
   • Qualitative or quantitive ELISA
   • Chemifluorescent immunoblotting
   • Potency assay

STERILITY ASSAYS
  
• USDA and FDA Standards